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ire1 reporter plhcx xbp1 mneongreen nls  (Addgene inc)


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    Addgene inc ire1 reporter plhcx xbp1 mneongreen nls
    Ire1 Reporter Plhcx Xbp1 Mneongreen Nls, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ire1 reporter plhcx xbp1 mneongreen nls/product/Addgene inc
    Average 93 stars, based on 3 article reviews
    ire1 reporter plhcx xbp1 mneongreen nls - by Bioz Stars, 2026-03
    93/100 stars

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    Figure 3. Flow cytometric determination of the level of <t>XBP1</t> <t>(mNeonGreen)</t> protein in heat-stressed U2OS cells. Cells were heat-stressed at 40 ◦C or 42 ◦C for one hour, followed by six hours of recovery at 37 ◦C. The Kolmogorov–Smirnov test was performed to analyze the equality of distributions. Samples treated at 40 or 42 ◦C differed from the control (37 ◦C) significantly (p < 0.05).
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    plhcx xbp1 mneongreen plasmid nls  (Addgene inc)
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    Figure 3. Flow cytometric determination of the level of <t>XBP1</t> <t>(mNeonGreen)</t> protein in heat-stressed U2OS cells. Cells were heat-stressed at 40 ◦C or 42 ◦C for one hour, followed by six hours of recovery at 37 ◦C. The Kolmogorov–Smirnov test was performed to analyze the equality of distributions. Samples treated at 40 or 42 ◦C differed from the control (37 ◦C) significantly (p < 0.05).
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    Figure 3. Flow cytometric determination of the level of XBP1 (mNeonGreen) protein in heat-stressed U2OS cells. Cells were heat-stressed at 40 ◦C or 42 ◦C for one hour, followed by six hours of recovery at 37 ◦C. The Kolmogorov–Smirnov test was performed to analyze the equality of distributions. Samples treated at 40 or 42 ◦C differed from the control (37 ◦C) significantly (p < 0.05).

    Journal: Cells

    Article Title: Mild Hyperthermia-Induced Thermogenesis in the Endoplasmic Reticulum Defines Stress Response Mechanisms.

    doi: 10.3390/cells13131141

    Figure Lengend Snippet: Figure 3. Flow cytometric determination of the level of XBP1 (mNeonGreen) protein in heat-stressed U2OS cells. Cells were heat-stressed at 40 ◦C or 42 ◦C for one hour, followed by six hours of recovery at 37 ◦C. The Kolmogorov–Smirnov test was performed to analyze the equality of distributions. Samples treated at 40 or 42 ◦C differed from the control (37 ◦C) significantly (p < 0.05).

    Article Snippet: To construct the U2OS cell line expressing fluorescently labeled XBP1 (mNeonGreen), 200 ng linearized pLHCX-XBP1 mNeonGreen plasmid NLS (Addgene plasmid #115971; http://n2t.net/addgene:115971; RRID: Addgene_115971), a gift from David Andrews [23], was transfected into 106 U2OS cells using the Amaxa Nucleofector System with the Cell Line Nucleofector Kit V (catalog no: VCA-1003, Lonza, Cologne, Germany) utilizing program X-001 according to the manufacturer’s protocol.

    Techniques: Control